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Product Description
GLI1 Antibody | 15-663 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human GLI1 (NP_001153517.1) .
Research Area: Cancer, Cell Cycle, Neuroscience, Signal Transduction, Stem Cell, Transcription
Tested Application: WB, IHC, IF
Application: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Specificiy: N/A
Positive Control 1: DU145
Positive Control 2: Mouse liver
Positive Control 3: mouse brain
Positive Control 4: rat brain
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Observed: 130kDa/160kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: GLI1, GLI
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
