Product Description
NEK3 Antibody | 22-391 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 267-506 of human NEK3 (NP_689933.1) .
Research Area: Cancer, Cell Cycle, Signal Transduction
Tested Application: WB, IF
Application: WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Specificiy: N/A
Positive Control 1: MCF7
Positive Control 2: HL-60
Positive Control 3: THP-1
Positive Control 4: HepG2
Positive Control 5: HeLa
Positive Control 6: Mouse kidney
Molecular Weight: Observed: 55kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: NEK3, HSPK36, MGC29949
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.