Product Description
RAD52 Antibody | 18-930 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 125-418 of human RAD52 (NP_602296.2) .
Research Area: Cell Cycle
Tested Application: WB, IHC
Application: WB: 1:500 - 1:1000
IHC: 1:50 - 1:200
Specificiy: N/A
Positive Control 1: HeLa
Positive Control 2: HL-60
Positive Control 3: Mouse thymus
Positive Control 4: Rat thymus
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Observed: 46kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: RAD52, RAD52 (S. cerevisiae) homolog, RAD52 homolog (S. cerevisiae) , Recombination protein RAD52
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.