Product Description
SENP6 Antibody | 18-038 | ProSci
Host: Rabbit
Reactivity: Rat
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 811-1112 of human SENP6 (NP_056386.2) .
Research Area: Cell Cycle
Tested Application: WB
Application: WB: 1:500 - 1:2000
Specificiy: N/A
Positive Control 1: Rat thymus
Positive Control 2: Rat spleen
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Observed: 126kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: SENP6, SUMO1/sentrin specific peptidase 6, SUSP1, SSP1, SENP6, KIAA0797, SUMO-1-specific protease, FKSG6
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Ubiquitin-like molecules (UBLs) , such as SUMO1 (UBL1; MIM 601912) , are structurally related to ubiquitin (MIM 191339) and can be ligated to target proteins in a similar manner as ubiquitin. However, covalent attachment of UBLs does not result in degradation of the modified proteins. SUMO1 modification is implicated in the targeting of RANGAP1 (MIM 602362) to the nuclear pore complex, as well as in stabilization of I-kappa-B-alpha (NFKBIA; MIM 164008) from degradation by the 26S proteasome. Like ubiquitin, UBLs are synthesized as precursor proteins, with 1 or more amino acids following the C-terminal glycine-glycine residues of the mature UBL protein. Thus, the tail sequences of the UBL precursors need to be removed by UBL-specific proteases, such as SENP6, prior to their conjugation to target proteins (Kim et al., 2000 [PubMed 10799485]) . SENPs also display isopeptidase activity for deconjugation of SUMO-conjugated substrates (Lima and Reverter, 2008 [PubMed 18799455]) .[supplied by OMIM, Jun 2009]