Product Description
COMT Antibody | 45-416 | ProSci
Host: Goat
Reactivity: Human
Homology: N/A
Immunogen: The immunogen for this antibody is: C-QDIIPQLKKKYDVD
Research Area: Cancer
Tested Application: E, WB, EIA, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 28kDa band in Human Testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and 24.5kDa according to NP_009294.1) . Recommended concentration: 0.03-0.1ug/ml.Immunohistochemistry:In paraffin embedded Human Brain (Cerebellum) shows staining of processes coming from the edge of the granular zone into the molecular layer. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Testis and Small Intestine. Recommended concentration: 3.75ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant COMTcaptured by a rabbit antibody. Recommended reporter concentration: 0.5-2ug/ml.
Specificiy: This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1)
Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: COMT, catechol-O-methyltransferase
User Note: N/A
BACKGROUND: N/A