Product Description
SOD1 Antibody | 46-412 | ProSci
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen: The immunogen for this antibody is: C-SRKHGGPKDEERH
Research Area: Cancer
Tested Application: E, WB, EIA
Application: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 18kDa band observed in Mouse Brain and Rat Spinal Cord lysates, and in lysates of cell line NIH3T3. Approx 20kDa observed in lysates of cell lines HEK293, HepG2 and MCF7 (calculated MW of 15.9kDa according to Human NP_000445.1, Mouse NP_035564.1 and Rat NP_058746.1) . Recommended concentration: 0.01-0.03µg/ml. Primary incubation 1 hour at room temperature. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant SOD1 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml
Specificiy: N/A
Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: SOD1, superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult) ) , HGNC:11179, ALS, ALS1, IPOA, SOD, Cu /Zn superoxide dismutase, Cu/Zn superoxide dismutase, SOD, soluble, indophenoloxidase A, superoxide dismutase (aa 120-154) , superoxide dismutase 1, soluble, superoxide dismutase, cystolic
User Note: N/A
BACKGROUND: N/A