Product Description
C3 Antibody | 43-098 | ProSci
Host: Goat
Reactivity: Human
Homology: N/A
Immunogen: The immunogen for this antibody is: QQRTFISPIKCRE
Research Area: Other
Tested Application: E, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 125kDa band in Human Liver lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kDa according to the largest fragment C3 alpha chain (aa672-1663) . Although this fragment is glycosylated, we have not yet found support in the literature for the observed size. The 125kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta and Testis. Recommended concentration: 5ug/ml.
Specificiy: This antibody is expected to recognize the C3 alpha chain, C3b alpha' chain and the C3c alpha' chain fragment 2.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Approx 125 kDa
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: C3, complement component 3, AHUS5, ARMD9, ASP, CPAMD1, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, OTTHUMP00000197086, acylation-stimulating protein cleavage product, complement C3, complement component C3
User Note: N/A
BACKGROUND: N/A