Product Description
CSX1 Antibody | 42-012 | ProSci
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Rat, Cow, Dog
Immunogen: The immunogen for this antibody is: C-PRAYSDPDPAKDPR
Research Area: Other
Tested Application: E, WB, IF
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40-45kDa band observed in Mouse Heart lysates (calculated MW of 34.2kDa according to NP_032726.1) . Preliminary experimants also showed an apprix 45kDa band in Human and Rat Heart kysates. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79 (5) :920-9.; PMID: 8888684) . Recommended concentration: 0.1-0.5ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells. Recommended concentration: 10ug/ml.
Specificiy: This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1) .
Positive Control 1: Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: NKX2-5, NK2 transcription factor related, locus 5 (Drosophila) , CHNG5, CSX, CSX1, NKX2.5, NKX2E, NKX4-1, NK2 transcription factor homolog E, NK2 transcription factor related, locus 5, cardiac-specific homeo box, tinman paralog
User Note: N/A
BACKGROUND: N/A