Product Description
DCX Antibody | 42-409 | ProSci
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen: The immunogen for this antibody is: C-KTSANMKAPQS
Research Area: Other
Tested Application: E, WB, IHC, IF
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45kDa band observed in Mouse fetal Brain lysates (calculated MW of 49.3kDa according to Mouse NP_001103692.1) . Recommended concentration: 0.01-0.03ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Cerebellum shows axonal and incidental nuclear staining. Recommended concentration: 2-4ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 5ug/ml.
Specificiy: This antibody is expected to recognize all four reported isoforms (NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1) . Reported variants represent identical protein: NP_835364.1 and NP_835366.1.
Positive Control 1: Cat. No. 1403-0 - Mouse Brain Tissue Lysate (0 Day Old)
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: DCX, doublecortin, RP5-914P14.1, DBCN, DC, FLJ51296, LISX, SCLH, XLIS, OTTHUMP00000023860, OTTHUMP00000216316, doublecortex, doublin, lis-X, lissencephalin-X, neuronal migration protein doublecortin
User Note: N/A
BACKGROUND: N/A