Product Description
EIF2B4 Antibody | 42-814 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen: The immunogen for this antibody is: C-RKDYGSKVSLFSH
Research Area: Other
Tested Application: E, WB
Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in lysates of cell line K562 (calculated MW of 59.6kDa according to NP_751945.2) . Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificiy: This antibody is expected to recognize all reported isoforms (NP_751945.2; NP_001029288.1; NP_056451.3) .
Positive Control 1: Cat. No. 1204 - K562 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: EIF2B4, eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa, DKFZp586J0119, EIF-2B, EIF2B, EIF2Bdelta, eIF-2B GDP-GTP exchange factor subunit delta, eukaryotic translation initiation factor 2B, subunit 4 delta, translation initiation factor eIF-2b delta subunit
User Note: N/A
BACKGROUND: N/A