Product Description
Hdac1 Antibody | 43-003 | ProSci
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Rat, Pig, Cow
Immunogen: The immunogen for this antibody is: C-PEDAIPEESGDE
Research Area: Other
Tested Application: E, WB, ChIP
Application: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 55kDa band observed in lysates of cell line NIH3T3 (calculated MW of 55.1kDa according to Human NP_032254.1 and Mouse NP_032254.1) . Recommended concentration: 0.3-1ug/ml.Chromatin Immunoprecipitation: A 4-fold increased presence of H3 onto the Human P21 locus observed when comparing the precipitations using EB11428 with non-specific goat IgG to bring down HDAC1 in a chromatin lysate from MCF7 cells.
Specificiy: This antibody may cross-react to HDAC2
Positive Control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Approx 55 kDa
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: HDAC1, histone deacetylase 1, RP23-209C6.7, HD1, Hdac1-ps, MommeD5, RPD3, MGC102534, MGC118085, OTTMUSP00000010171
User Note: N/A
BACKGROUND: N/A