Product Description
Liver X Receptor Antibody | 45-100 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen: The immunogen for this antibody is: CRLQDKKLPPLLSEI
Research Area: Homeostasis
Tested Application: E, WB
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Western blot: Approx 55kDa band observed in Human, Liver and Kidney lysates and in preliminary testing of Human Spleen lysate (calculated MW of 51.1kDa according to NP_001238863.1) . This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on NIH3T3 and Rat for this particular batch. Immunofluorescence:Strong expression of the protein seen in the nuclei of A549 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
Specificiy: This antibody is expected to recognise an epitope corresponding to aa 429-442 of human LXR alpha protein (NP_005684.2) and to aa 443-456 of human LXR beta protein (NP_009052.3) .
Positive Control 1: Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: NR1H3, LXRA, LXR-a, RLD-1, nuclear receptor subfamily 1, group H, member 3, liver X receptor, alpha, NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor / NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor, OTTHUMP00000198038, liver X receptor alpha, liver X receptor-alpha, LXRB, LX receptor beta, liver X receptor beta, liver X receptor-beta, nuclear orphan receptor LXR-beta, oxysterols receptor LXR-beta
User Note: N/A
BACKGROUND: N/A