Product Description
MKRN1 Antibody | 42-850 | ProSci
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen: The immunogen for this antibody is: C-RYEHSKPLKQEEAT
Research Area: Other
Tested Application: E, WB
Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50kDa band observed in fetal Mouse Brain lysates and 35kDa plus fainter 50kDa bands in adult Mouse Brain lysates (calculated MW of 52.9kDa according to NP_061280.2) . The observed 35kDa band corresponds to the calculated MW of human isoform 2 (35.2kDa according to NP_001138597.1) , but this size isoform has not yet been confirmed in Mouse. Recommended concentration: 1-3ug/ml. In transfected HEK293 transiently expressing full-length Human MKRN1 (myc and DYKDDDDK tagged) , a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.3- 1ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.
Specificiy: This antibody is expected to recognize both reported isoforms (NP_038474.2; NP_001138597.1) .
Positive Control 1: Transfected HEK293
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: MKRN1, makorin ring finger protein 1, FLJ21334, RNF61, E3 ubiquitin-protein ligase makorin-1, OTTHUMP00000212121, OTTHUMP00000212124, RING finger protein 61
User Note: N/A
BACKGROUND: N/A