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Product Description
MTHFD1L Antibody | 42-396 | ProSci
Host: Goat
Reactivity: Human, Mouse, Rat
Homology: Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen: The immunogen for this antibody is: C-DTNDRFLRKITIGQ
Research Area: Other
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110kDa band observed in Human Brain (Cerebellum) (calculated MW of 106kDa according to NP_056255.2) . The same band is observed in lysates of cell line NIH3T3, Mouse Brain and Rat Brain. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig for this particular batch.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Specificiy: N/A
Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: MTHFD1L, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like, DKFZp586G1517, FLJ21145, FTHFSDC1, MTC1THFS, dJ292B18.2, 10-formyl-THF synthetase, formyltetrahydrofolate synthetase domain containing 1, mitochondrial C1-tetrahydrofolate synthase, mitochondrial C1-tetrahydrofolate synthetase, monofunctional C1-tetrahydrofolate synthase, mitochondrial
User Note: N/A
BACKGROUND: N/A
