Product Description
MYO5A Antibody | 42-827 | ProSci
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen: The immunogen for this antibody is: C-ETKQLELDLN
Research Area: Other
Tested Application: E, WB, IF
Application: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 200kDa band observed in lysates of cell line Jurkat (calculated MW of 215kDa according to NP_000250.3) . Recommended concentration: 1-3ug/ml.Immunofluorescence: Transverse sections of Mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (Röder et al, Proc Natl Acad Sci U S A. 2010 Feb 2;107 (5) :2031-6; PMID: 20133847) . Recommended concentration: 1-3ug/ml.
Specificiy: This antibody is expected to recognize both reported isoforms (NP_000250.3; NP_001135967.1) .
Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: MYO5A, myosin VA (heavy chain 12, myoxin) , GS1, MYH12, MYO5, MYR12, dilute, myosin V, myosin VA, myosin, heavy polypeptide kinase, myoxin
User Note: N/A
BACKGROUND: N/A