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Product Description
SPI1 Antibody | 42-746 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Rat
Immunogen: The immunogen for this antibody is: C-DLYQRQTHEYY
Research Area: Other
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2kDa according to NP_001074016.1 and of 31.1kDa according to NP_003111.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Harendza S et al, J Biol Chem. 2000 Jun 30;275 (26) :19552-9.; PMID: 10867017) . Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen and Colon (lymphoid aggregate) . Recommended concentration: 3.75ug/ml.
Specificiy: This antibody is expected to recognise both reported isoforms (NP_001074016.1; NP_003111.2) .
Positive Control 1: Cat. No. 1224 - Daudi Cell Lysate
Positive Control 2: Cat. No. 1204 - K562 Cell Lysate
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: PU.1, SPI1, spleen focus forming virus (SFFV) proviral integration oncogene spi1, hCG_25181, SFPI1, SPI-1, SPI-A, 31 kDa transforming protein, SPI-1 proto-oncogene, hematopoietic transcription factor PU.1
User Note: N/A
BACKGROUND: N/A
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