Product Description
PDL1 Recombinant Protein | 96-596 | ProSci
Tested Application: WB
Application: This recombinant protein can be used for WB. For research use only.
Tpredicted Molecular Weight: 26 kDa
Physical State: Lyophilized
Buffer: PBS, pH7.4
Storage Condition: Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Alternate Name: PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1
Background: Dong H., et al., 1999, Nat. Med. 5:1365-1369.
Shipping: N/A
Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
Source: HEK293 cells
Species: Human
By Source: Human Cells
By Species: Human
Fusion Tag: His Tag
Sequence: Phe 19 - Arg 238
Bological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human PDL1 /CD274 Protein at 5μg/mL (100 μl/well),can bind Biotinylated Human PDCD1 Protein, C-Fc, His & AVI Tagged. The EC50 is typically 0. 16-2. 4μg/mL. Measured by its binding ability in a functional ELISA. Immobilized rh PD-L1 / B7-H1 at 2μg/ml (100μl/well) can bind rhPD1/PDCD1 Fc Chimera with a linear range of 0. 05-1μg/ml. Measured by its ability to inhibit antiCD3 antibody induced IL2 secretion in human T lymphocytes. The ED50 for this effect is typically 0. 6-5μg/ml.
Purity: >98% as determined by SEC-HPLC and SDS-PAGE.