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Product Description
FKBP12 Antibody | 7789 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%) , Rabbit: (100%)
Immunogen: FKBP12 antibody was raised against an 18 amino acid peptide near the center of human FKBP12.
The immunogen is located within the first 50 amino acids of FKBP12.
Research Area: Homeostasis, Immunology
Tested Application: E, WB, ICC, IF
Application: FKBP12 antibody can be used for detection of FKBP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: FKBP12 antibody is human, mouse and rat reactive.
Positive Control 1: Cat. No. 1202 - A431 Cell Lysate
Positive Control 2: Cat. No. 17-002 - A-431 Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 12 kDa
Observed: 12 kDa
Validation: N/A
Isoform: N/A
Purification: FKBP12 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: FKBP12 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: FKBP12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: FKBP12 Antibody: FKBP1, PKC12, PKCI2, FKBP12, PPIASE, FKBP-12, FKBP-1A, FKBP1, Peptidyl-prolyl cis-trans isomerase FKBP1A, 12 kDa FK506-binding protein, PPIase FKBP1A
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: FKBP, or FK506 binding protein, is a family of proteins that have prolyl isomerase activity and are related to the cyclophilins in function (1) . FKBP12, also called FKBP1, was originally identified as a target of FK506 and rapamycin activity and catalyzes the transition between cis and transproline residues critical for proper folding of proteins (2) . FKBP12 is an abundant, evolutionarily conserved cytoplasmic protein that is an important regulator of diverse array of cellular processes including T cell activation, entry into the cell cycle and intracellular calcium release (3-5) .
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