Product Description
GJB2 Antibody | 8031 | ProSci
Host: Rabbit
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Rat: (94%) , Mouse: (94%) , Sheep: (88%) , Bovine: (88%) , Chicken: (82%)
Immunogen: GJB2 antibody was raised against a 16 amino acid peptide near the center of human GJB2.
The immunogen is located within amino acids 80 - 130 of GJB2.
Research Area: Homeostasis
Tested Application: E, WB
Application: GJB2 antibody can be used for detection of GJB2 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificiy: GJB2 antibody is human specific.
Positive Control 1: Cat. No. 1320 - Human Colon Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 25 kDa
Observed: 26 kDa
Validation: N/A
Isoform: N/A
Purification: GJB2 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: GJB2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: GJB2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Gap junction beta-2 protein, connexin 26, CX26, DFNA3, DFNA3A, DFNB1, DFNB1A, HID, KID, NSRD1, PPK
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The Gap junction beta-2 protein (GJB2) , also known as Connexin 26, is member of the gap junction protein family which form structures that were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells (1) . Mutations in the GJB2 gene are thought to be responsible for as much as 35-45% of congenital sensorineural hearing loss in some populations (2) . Other mutations in this gene have also been linked to a wide array of skin diseases (3) .