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Product Description
GOLGA5 Antibody | 7387 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: GOLGA5 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human GOLGA5.
The immunogen is located within amino acids 540 - 590 of GOLGA5.
Research Area: Homeostasis
Tested Application: E, WB, IHC-P, IF
Application: GOLGA5 antibody can be used for detection of GOLGA5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: GOLGA5 antibody is human, mouse and rat reactive. Two isoforms of GOLGA5 exist due to alternative splicing events.
Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2: Cat. No. 11-701 - Human Testis Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 64, 80 kDa
Observed: 65, 78 kDa
Validation: N/A
Isoform: N/A
Purification: GOLGA5 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: GOLGA5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: GOLGA5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: GOLGA5 Antibody: RFG5, GOLIM5, ret-II, RETII, RFG5, PIG31, Golgin subfamily A member 5, Cell proliferation-inducing gene 31 protein
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: GOLGA5 Antibody: GOLGA5 (Golgi autoantigen, subfamily A, member 5) , also known as GOLGA84, RFG5 (RET-fused gene 5 protein) or GOLIM5, is a ubiquitously expressed single-pass type II coiled-coil membrane protein that is involved in maintaining Golgi structure (1) . It is localized to the Golgi apparatus and predominantly found on membranes at the cis side of the Golgi stack (1, 2) . As a mitotic phosphoprotein, GOLGA5 binds to active Rab 1 and associates with CASP (an isoform of CDP) in a golgin-tethering complex that is believed to play a role in intra-Golgi retrograde transport (3, 4) . Defects in GOLGA5 are a cause of thyroid papillary carcinoma (TPC) (5) .
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