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Product Description
RNF8 Antibody | 5763 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (92%)
Immunogen: RNF8 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human RNF8.
The immunogen is located within amino acids 330 - 380 of RNF8.
Research Area: Homeostasis
Tested Application: E, WB, IF
Application: RNF8 antibody can be used for detection of RNF8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificiy: At least three isoforms of RNF8 are known to exist; this antibody will detect all three isoforms.
Positive Control 1: Cat. No. 1302 - Human Lung Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 46, 53 kDa
Observed: 50 kDa
Validation: N/A
Isoform: N/A
Purification: RNF8 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: RNF8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: RNF8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: RNF8 Antibody: hRNF8, KIAA0646, E3 ubiquitin-protein ligase RNF8, RING finger protein 8, hRNF8
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: RNF8 Antibody: RNF8 was identified as a ubiquitin ligase (E3) containing a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes including UBE2E1/UBCH6, UBE2E2, and UBE2E3. RNF8 assembles at DNA double-strand breaks (DSBs) via interactions though the FHA domain with the adaptor protein MDC1, resulting in an increase in DSB-associated H2A histone ubiquitinations mediated by the associated ubiquitin ligase RNF168 followed by the accumulation of 53BP1 and BRCA1 repair proteins. Together with RNF168, RNF8 plays an integral part of class switch recombination in B cells, allowing the production of several classes of antibodies, through the recruitment of 53BP1 and BRCA1 to the DSB sites.
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