Product Description
SCARA5 Antibody | 5259 | ProSci
Host: Rabbit
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%) , Mouse: (88%) , Chicken: (86%) , Rat: (80%)
Immunogen: SCARA5 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SCARA5.
The immunogen is located within amino acids 350 - 400 of SCARA5.
Research Area: Homeostasis
Tested Application: E, WB, ICC, IF
Application: SCARA5 antibody can be used for detection of SCARA5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: At least four isoforms of SCARA5 are known to exist; this antibody will detect all four isoforms.
Positive Control 1: Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2: Cat. No. 17-011 - Hep G2 Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 24, 39, 44, 54 kDa
Observed: 50 kDa
Validation: N/A
Isoform: N/A
Purification: SCARA5 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: SCARA5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: SCARA5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: SCARA5 Antibody: Tesr, NET33, Scavenger receptor class A member 5, Scavenger receptor hlg
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: SCARA5 Antibody: Scavenger receptor class A member 5 (SCARA5) , also known as TESR, is part of the scavenger receptor superfamily, which is composed of many members with diverse structures, expression patterns, and functions. SCARA5 is a cell-surface receptor that contains the collagen-like domain and N-terminal scavenger receptor cysteine rich domain typical of the class A scavenger receptor subfamily. Unlike other class A receptors, SCARA5 does not endocytose acetylated or oxidized low density lipoprotein. Instead, SCARA5 binds serum ferritin and mediates its endocytosis from the cell surface, indicating SCARA5 may play a role in non-transferrin iron delivery.