Product Description
SKI2W Antibody | 7993 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: SKI2W antibody was raised against a 16 amino acid peptide near the amino terminus of human SKI2W.
The immunogen is located within amino acids 60 - 110 of SKI2W.
Research Area: Homeostasis
Tested Application: E, WB, IHC-P, IF
Application: SKI2W antibody can be used for detection of SKI2W by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: SKI2W antibody is human, mouse and rat reactive. At least two isoforms of SKI2W are known to exist; this antibody will detect both isoforms.
Positive Control 1: Cat. No. 1415 - Mouse Stomach Tissue Lysate
Positive Control 2: Cat. No. 10-801 - Human Stomach Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 113, 137 kDa
Observed: 135 kDa
Validation: N/A
Isoform: N/A
Purification: SKI2W antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: SKI2W antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: SKI2W antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Superkiller viralicidic activity 2-like, DDX13, Helicase-like protein, Helicase SKI2W, HLP, SKI2, SKIV2, SKIV2L
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: SKI2W (SKIV2L, Helicase-like protein) , a DEVH-box protein, is a component of the SKI complex which is involved in the regulation of translation and RNA turnover (1, 2) . SKI2W is a nucleolar and cytoplasmic protein that has a putative RNA helicase domain (2) . The potential roles of Ski2W on the clearance of degraded nuclear and cytoplasmic RNA raised their possibilities as susceptibility genes of systemic lupus erythematosus (3, 4) .