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Product Description
SMARCA4 Antibody | 7749 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen: SMARCA4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human SMARCA4.
The immunogen is located within amino acids 1420 - 1470 of SMARCA4.
Research Area: Homeostasis
Tested Application: E, WB, IHC-P, IF
Application: SMARCA4 antibody can be used for detection of SMARCA4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificiy: SMARCA4 antibody is human, mouse and rat reactive. Multiple isoforms of SMARCA4 are known to exist.
Positive Control 1: Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 185 kDa
Observed: 200 kDa
Validation: N/A
Isoform: N/A
Purification: SMARCA4 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: SMARCA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: SMARCA4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: SMARCA4 Antibody: BRG1, SNF2, SWI2, MRD16, RTPS2, BAF190, SNF2L4, SNF2LB, hSNF2b, BAF190A, BRG1, SNF2B, Transcription activator BRG1, ATP-dependent helicase SMARCA4
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 4 (SMARCA4) protein, also known as BRG1, is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila (1) . Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes (2) . The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (3) . In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44 (4, 5) .
