Product Description
SPT1 Antibody | 6303 | ProSci
Host: Rabbit
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Mouse: (88%) , Bovine: (76%)
Immunogen: SPT1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SPT1.
The immunogen is located within amino acids 380 - 430 of SPT1.
Research Area: Homeostasis
Tested Application: E, WB, IHC-P
Application: SPT1 antibody can be used for detection of SPT1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificiy: At least two isoforms of SPT1 are known to exist; this antibody will detect only isoform a. SPT1 antibody is predicted to not cross-react with SPT2.
Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2: Cat. No. 1203 - A549 Cell Lysate
Positive Control 3: Cat. No. 10-101 - Human Lung Tissue Slide
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 51 kDa
Observed: 51 kDa
Validation: N/A
Isoform: N/A
Purification: SPT1 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: SPT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: SPT1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: SPT1 Antibody: HSN1, LBC1, LCB1, SPT1, SPTI, HSAN1, Serine palmitoyltransferase 1, Long chain base biosynthesis protein 1, LCB 1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: SPT1 Antibody: Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT1 is the long chain base subunit 1 of mammalian serine palmitoyltransferase. SPT1 is not catalytically active but is necessary for the stabilization of the SPT2 subunit and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. Missense mutations in this gene have been identified in patients with hereditary sensory neuropathy type 1 (HSAN1) . These mutations induce a shift in the substrate specificity of the holoenzyme, leading to the formation and accumulation of two neurotoxic sphingolipids.