Product Description
TEM4 Antibody | 4367 | ProSci
Host: Rabbit
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Mouse: (94%)
Immunogen: TEM4 antibody was raised against a 18 amino acid synthetic peptide near the center of human TEM4.
The immunogen is located within amino acids 910 - 960 of TEM4.
Research Area: Homeostasis
Tested Application: E, WB, IHC-P
Application: TEM4 antibody can be used for detection of TEM4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 220 kDa
Observed: 200 kDa
Validation: N/A
Isoform: N/A
Purification: TEM4 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: TEM4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: TEM4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: TEM4 Antibody: TEM4, P164RHOGEF, p164-RhoGEF, KIAA0337, TEM4, Rho guanine nucleotide exchange factor 17, 164 kDa Rho-specific guanine-nucleotide exchange factor
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: TEM4 Antibody: Rho GTPases, which are activated by specific guanine-nucleotide exchange factors (GEFs) , play pivotal roles in several cellular functions. TEM4, encoding a protein containing 1510 amino acids, contains a RhoGEF-specific Dbl homology (DH) domain but lacks their typical pleckstrin homology domain. TEM4 is a Rho-specific GEF with novel structural and regulatory properties and predominant expression in the heart. It couples tyrosine kinase signals with the activation of the rho/rac GTPases, thus leading to cell differentiation and/or proliferation. Elevated levels of TEM4, TEM5, TEM6, TEM7 and TEM7R were also raised in breast cancer tissues. TEM4 could also prove to be useful targets therapeutically.