Product Description
TEM5 Antibody | 4371 | ProSci
Host: Rabbit
Reactivity: Human, Rat
Homology: Predicted species reactivity based on immunogen sequence: Mouse: (85%)
Immunogen: TEM5 antibody was raised against a 14 amino acid synthetic peptide near the center of human TEM5.
The immunogen is located within amino acids 560 - 610 of TEM5.
Research Area: Homeostasis
Tested Application: E, WB, IHC-P, IF
Application: TEM5 antibody can be used for detection of TEM5 by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1465 - Rat Kidney Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: TEM5 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: TEM5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: TEM5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: TEM5 Antibody: TEM5, KIAA1531, TEM5, G-protein coupled receptor 124, Tumor endothelial marker 5
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: TEM5 Antibody: Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogensis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. These include TEMs such as TEM1, TEM5, TEM7 and TEM8. TEM5 is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. TEM5 is a seven-pass transmembrane receptor, unlike TEM1, TEM7 and TEM8 which span the membrane once. TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.