Product Description
TYW3 Antibody | 6377 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%) , Pig: (94%)
Immunogen: TYW3 antibody was raised against a 17 amino acid synthetic peptide near the center of human TYW3.
The immunogen is located within amino acids 160 - 210 of TYW3.
Research Area: Homeostasis
Tested Application: E, WB
Application: TYW3 antibody can be used for detection of TYW3 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificiy: TYW3 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW3 are known to exist; this antibody will detect both isoforms.
Positive Control 1: Cat. No. 1203 - A549 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: TYW3 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: TYW3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: TYW3 antibody can be stored at 4 ˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: TYW3 Antibody: C1orf171, C1orf171, tRNA wybutosine-synthesizing protein 3 homolog, tRNA-yW-synthesizing protein 3
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: TYW3 Antibody: TYW3 is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW3 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation.