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Product Description
VAMP7 Antibody | 6929 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Chicken: (100%) , Bovine: (100%)
Immunogen: VAMP7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human VAMP7.
The immunogen is located within amino acids 20 - 70 of VAMP7.
Research Area: Homeostasis
Tested Application: E, WB, IF
Application: VAMP7 antibody can be used for detection of VAMP7 by Western blot at 0.5 - 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: At least three isoforms of VAMP7 are known to exist; this antibody will detect the two longest isoforms. This antibody is predicted to not cross-react with other Ras-related proteins.
Positive Control 1: Cat. No. 1402 - Mouse Lung Tissue Lysate
Positive Control 2: Cat. No. 10-101 - Human Lung Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 24, 29 kDa
Observed: 32 kDa
Validation: N/A
Isoform: N/A
Purification: VAMP7 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: VAMP7 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: VAMP7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: VAMP7 Antibody: SYBL1, TIVAMP, VAMP-7, TI-VAMP, SYBL1, Vesicle-associated membrane protein 7, Synaptobrevin-like protein 1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: VAMP7 Antibody: VAMP7 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family, localizing to late endosomes and lysosomes. VAMP7 is thought to mediate the fusion of endosomes to their target lysosomes as well as other exocytosis events during phagocytosis and neuritogenesis. VAMP7 interacts with the VPS9 ankyrin repeat protein VARP, a protein that localizes to early endosomes and thought to regulate endosome dynamics. Together with CD82, VAMP7 can modulate the signaling of EGFR by regulating its endocytosis from the plasma membrane.
