Product Description
ZBED1 Antibody | 5111 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: N/A
Immunogen: ZBED1 antibody was raised against a 15 amino acid synthetic peptide from near the center of human ZBED1.
The immunogen is located within amino acids 180 - 230 of ZBED1.
Research Area: Homeostasis
Tested Application: E, WB
Application: ZBED1 antibody can be used for detection of ZBED1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificiy: This antibody is predicted to have no cross-reactivity to ZBED2 or ZBED3.
Positive Control 1: Cat. No. 1203 - A549 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: ZBED1 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: ZBED1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: ZBED1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: ZBED1 Antibody: ALTE, DREF, TRAMP, hDREF, ALTE, KIAA0785, Zinc finger BED domain-containing protein 1, Putative Ac-like transposable element
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: ZBED1 Antibody: ZBED1, also known as DREF, is the human homolog of the Drosophila protein DREF, a transcriptional regulatory factor required for expression of genes involved in DNA replication and cell proliferation. RNA interference (RNAi) experiments targeting ZBED1 RNA resulted in the inhibition of S phase entry and reduction of histone H1 mRNA in HeLa cells, suggesting that ZBED1 also plays a role in cell proliferation in human cells. At least 22 ribosomal proteins (RPs) possess ZBED1 binding sites in their promoters; anti-ZBED1 RNAi also resulted in decreased RP gene expression. Immunoprecipitation analyses have shown that ZBED1 self-associates in vivo via a carboxyl-terminal hATC domain, and this self-association is required for nuclear localization and DNA binding.