Product Description
APP Antibody | 2136 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Pig: (100%) , Guinea pig: (100%)
Immunogen: APP antibody was raised against a 15 amino acid peptide near the carboxy terminus of human APP.
The immunogen is located within the last 50 amino acids of APP.
Research Area: Neuroscience
Tested Application: E, WB, IHC-P, IF
Application: APP antibody can be used for detection of APP and the C99 fragment by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 83 kDa
Observed: 110 kDa
Validation: N/A
Isoform: N/A
Purification: APP Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: APP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Condition: APP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: APP Antibody: AAA, AD1, PN2, ABPP, APPI, CVAP, ABETA, PN-II, CTFgamma, A4, Amyloid beta A4 protein, APP
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: APP Antibody: Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99) . The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta) , which is deposited in the brains of all suffers of Alzheimer's disease.