Product Description
BTLA Antibody | 7473 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: BTLA antibody was raised against a 17 amino acid peptide near the center of human BTLA.
The immunogen is located within amino acids 170 - 220 of BTLA .
Research Area: Innate Immunity
Tested Application: E, WB, IHC-P, IF
Application: BTLA antibody can be used for detection of BTLA by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: BTLA antibody is human specific. At least two isoforms of BTLA are known to exist.
Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2: Cat. No. 11-801 - Human Small Intestine Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 32 kDa
Observed: 40kDa
Validation: N/A
Isoform: N/A
Purification: BTLA Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: BTLA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: BTLA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: BTLA Antibody : BTLA1, CD272, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The B- and T-lymphocyte attenuator (BTLA) protein is a member of the immunoglobulin superfamily containing a single immunoglobulin (Ig) domain (1) . Like other coihibitory receptors such as CTLA-4 and PD-1, BTLA functions as a receptor that relays inhibitory signals to suppress the immune response, and can inhibit LPS-induced endotoxic shock by suppressing TLR4 signaling in innate immune cells (1, 2) . Polymorphisms in this gene have also been associated with an increased risk of rheumatoid arthritis (3) .