Product Description
DDC Antibody | 7447 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (92%) , Guinea pig: (77%) , Pig: (77%)
Immunogen: DDC antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DDC.
The immunogen is located within amino acids 390 - 440 of DDC .
Research Area: Neuroscience
Tested Application: E, WB, IHC-P, IF
Application: DDC antibody can be used for detection of DDC by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: DDC antibody is human, mouse and rat reactive.
Positive Control 1: Cat. No. 1302 - Human Lung Tissue Lysate
Positive Control 2: Cat. No. 10-101 - Human Lung Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 53 kDa
Observed: 53 kDa
Validation: N/A
Isoform: N/A
Purification: DDC Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: DDC Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: DDC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: DDC Antibody : AADC, AADC, Aromatic-L-amino-acid decarboxylase, DOPA decarboxylase
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: DOPA decarboxylase (DDC) belongs to the group II decarboxylase family of proteins (1) . It is an important protein in the catecholamine biosynthesis pathway. DDC catalyzes the second reaction in the biosynthesis of catecholamines, trace amines and serotonin (1, 2) . It can form a homodimer and is expressed in the central nervous system (2) . DDC can be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease, neuroendocrine tumors and drug abuse (3, 4) . Defects in DDC gene may cause the autosomal recessive disorder AADC deficiency (4) .