Product Description
GOLGIN97 Antibody | 8215 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: GOLGIN97 antibody was raised against a 17 amino acid peptide near the amino terminus of human GOLGIN97.
The immunogen is located within amino acids 30 - 80 of GOLGIN97.
Research Area: Homeostasis, Immunology
Tested Application: E, WB, IHC-P, IF
Application: GOLGIN97 antibody can be used for detection of GOLGIN97 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificiy: GOLGIN97 antibody is human, mouse and rat reactive. At least two isoforms of GOLGIN97 are known to exist; this antibody will detect only the longer isoform. GOLGIN97 antibody is predicted to not cross-react with other GOLGIN family proteins.
Positive Control 1: Cat. No. 1319 - Human Stomach Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 84 kDa
Observed: 120 kDa
Validation: N/A
Isoform: N/A
Purification: GOLGIN97 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: GOLGIN97 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: GOLGIN97 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Golgin subfamily A member 1, GOLGA1, GOLGIN A1, GOLGIN-97
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: GOLGIN97 was initially isolated as a Golgi complex autoantigen associated with Sjogren’s syndrome, a chronic autoimmune disease in which the body's white blood cells destroy the exocrine glands, specifically the salivary and lacrimal glands (1, 2) . GOLGIN97 plays a role as a tethering molecule in endosome to the trans-Golgi network (TGN) retrograde traffic (3) . FIP1/RCP binding to GOLGIN97 is required for tethering and fusion of recycling endosome retrograde transport vesicles to the TGN (4) .