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Product Description
IL-17 Antibody | 4887 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (84%) , Rat: (81%) , Pig: (74%)
Immunogen: Anti-IL-17 antibody (4887) was raised against a peptide corresponding to 16 amino acids near the center of mature human IL-17.
The immunogen is located within amino acids 50-100 of IL-17.
Research Area: Signal Transduction
Tested Application: E, WB, IHC-P, IF
Application: WB: 0.125-2 μg/mL; IF: 5-20 μg/mL; IHC: 1-5 μg/mL .
Antibody validated: Western Blot in human samples; Immunofluorescence in mouse samples; Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 17-208 - A20 Cell Slide
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 17.5kD
Observed: 18 kDa
Validation:
Recombinant Protein Test (Figure 1) : Anit-IL-17 antibodies (4887) detected human IL-17 recombinant protein at different concentrations
Isoform: Human IL-17 has only one isoform (155aa, 17.5kD) . Mouse IL-17 has one isoform (158aa, 17.5kD) and Rat IL-17 also has one isoform (150aa, 16.9kD) . 4887 can detect human, mouse and rat IL-17.
Purification: IL-17 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: IL-17 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: IL-17 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: IL-17 Antibody: IL17, CTLA8, IL-17, IL-17A, IL17, Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: IL-17 Antibody: Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-κB and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.
