Product Description
Slug Antibody | 3959 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%) , Rat: (92%)
Immunogen: Slug antibody was raised against a 13 amino acid synthetic peptide from near the center of human Slug.
The immunogen is located within amino acids 90 - 140 of Slug.
Research Area: Signal Transduction, Cancer
Tested Application: E, WB, IF
Application: Slug antibody can be used for detection of Slug by Western blot at 0.5 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: This Slug antibody is predicted to not cross-react with Snail.
Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2: Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 3: Cat. No. 10-401 - Human Kidney Tissue Slide
Positive Control 4: Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 29 kDa
Observed: 30 kDa
Validation: N/A
Isoform: N/A
Purification: Slug Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Slug Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: Slug antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: Slug Antibody: SLUG, WS2D, SLUGH1, SNAIL2, SLUG, SLUGH, Zinc finger protein SNAI2, Neural crest transcription factor Slug
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Slug Antibody: Slug, a member of the Snail family of C2H2-type zinc finger transcription factors, was initially identified to be involved in epithelial-mesenchymal transitions as well as the formation of the neural tube during vertebrate embryogenesis. Like Snail, Slug transcription can be induced by growth factors such as FGF, BMP, and TGF-beta. Once expressed, Slug will bind E-box regions of promoters and repress transcription of genes such as E-cadherin and Claudin-. More recently, its expression in breast, esophogeal, and colorectal carcinomas has been correlated with poor prognosis for survival. Furthermore, Slug can protect hemapoietic progenitor cells from radiation-induced apoptosis by repressing the p53-mediated transcription of Puma, a BH3-only antagonist of the anti-apoptotic members of the Bcl-2 family.