Product Description
SPINSTER Antibody | 7651 | ProSci
Host: Rabbit
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (83%) , Rat: (81%) , Mouse: (81%)
Immunogen: SPINSTER antibody was raised against an 18 amino acid peptide near the amino terminus of human SPINSTER.
The immunogen is located within the first 50 amino acids of SPINSTER.
Research Area: Autophagy
Tested Application: E, WB, IHC-P, IF
Application: SPINSTER antibody can be used for detection of SPINSTER by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: SPINSTER antibody is human specific. At least four isoforms of SPINSTER are known to exist. This antibody is predicted to not cross-react with other members of the spinster family of proteins.
Positive Control 1: Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2: Cat. No. 1309 - Human Placenta Tissue Lysate
Positive Control 3: Cat. No. 10-101 - Human Lung Tissue Slide
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 58 kDa
Observed: 56 kDa
Validation: N/A
Isoform: N/A
Purification: SPINSTER antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: SPINSTER antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: SPINSTER antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: SPINSTER Antibody: LAT, nrs, SPIN1, SPINL, HSpin1, PP2030, PP20300, Protein spinster homolog 1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: SPINSTER, also known as SPNS1 or SPIN1, is a 528 amino acid multi-pass membrane protein that localizes to the inner mitochondrial membrane and belongs to the spinster subfamily of the major facilitator superfamily (1) . SPINSTER interacts with Bcl-x and Bcl-2 and, via this interaction, is thought to be involved in necrotic or autophagic cell death (2) . The related protein SPNS2 is critical for the normal lymphocyte localization and mammalian immune system function (1, 3) .