Product Description
ZBP1 Antibody | 4401 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: ZBP1 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human ZBP1.
The immunogen is located within the last 50 amino acids of ZBP1.
Research Area: Innate Immunity
Tested Application: E, WB, IHC-P, IF
Application: ZBP1 antibody can be used for the detection of ZBP1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: Multiple isoforms of ZBP1 are known to exist.
Positive Control 1: Cat. No. 1408 - Mouse Small Intestine Tissue Lysate
Positive Control 2: Cat. No. 11-801 - Human Small Intestine Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 39, 45 kDa
Observed: 42 kDa
Validation: N/A
Isoform: N/A
Purification: ZBP1 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: ZBP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: ZBP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: ZBP1 Antibody: DAI, DLM1, DLM-1, C20orf183, Z-DNA-binding protein 1, Tumor stroma and activated macrophage protein DLM-1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: ZBP1 Antibody: Z-DNA binding protein 1 (ZBP1) belongs to a family of proteins that contain the Zalpha domain which binds specifically to left-handed Z-DNA and Z-RNA. ZBP1 was initially identified as a novel gene that was up-regulated in activated macrophages in mice bearing ascites tumors, suggesting that is may play a role in processes such as host response in neoplasia. More recent reports indicate that the cytosolic ZBP1 has can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of ZBP1 to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK) .