Product Description
ZF21 Antibody | 6935 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (93%)
Immunogen: ZF21 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ZF21.
The immunogen is located within the first 50 amino acids of ZF21.
Research Area: Cancer, Cell Cycle
Tested Application: E, WB, IF
Application: ZF21 antibody can be used for detection of ZF21 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: At least two isoforms of ZF21 are known to exist; this antibody will detect both isoforms.
Positive Control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: ZF21 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: ZF21 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: ZF21 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: ZF21 Antibody: ZF21, Zinc finger FYVE domain-containing protein 21, ZF21
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: ZF21 Antibody: ZF21 was initially identified as protein that could bind to the cytoplasmic tail of MT1-MMP (Membrane-type 1 matrix metalloproteinase) , a potent invasion-promoting protease. ZF21 is a member of a protein family characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain and regulates focal adhesions (FAs) and cell movement. Knockdown of ZF21 expression resulted in a delay of FA disassembly following induction of synchronous disassembly of FAs by nocodazole treatment, suggesting that ZF21 is involved in FA disassembly. ZF21 contains a noncanonical pleckstrin homology domain that is a possible therapeutic target to treat metastatic cancer.