Product Description
ZIMP10 Antibody | 6237 | ProSci
Host: Rabbit
Reactivity: Human
Homology: Predicted species reactivity based on immunogen sequence: Mouse: (92%)
Immunogen: ZIMP10 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ZIMP10.
The immunogen is located within amino acids 90 - 140 of ZIMP10.
Research Area: Cancer, Cell Cycle
Tested Application: E, WB, ICC, IF
Application: ZIMP10 antibody can be used for detection of ZIMP10 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: At least three isoforms are known to exist; this antibody will recognize all three. ZIMP10 antibody is predicted to not cross-react with other PIAS protein family members.
Positive Control 1: Cat. No. 1204 - K562 Cell Lysate
Positive Control 2: Cat. No. 17-004 - K-562 Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: ZIMP10 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: ZIMP10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: ZIMP10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: ZIMP10 Antibody: MIZ, RAI17, ZIMP10, hZIMP10, TRAFIP10, KIAA1224, Zinc finger MIZ domain-containing protein 1, PIAS-like protein Zimp10
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: ZIMP10 Antibody: ZIMP10, also known as ZMIZ1, is a novel PIAS (protein inhibitor of activated signal transducer and activator of transcription) -like protein initially identified as a transcriptional co-activator of the androgen receptor (AR) . ZIMP10 and the related protein ZIMP7 interact with PIAS3 and enhances AR-mediated transcription. Later experiments showed that ZIMP10 is also a co-activator of the p53 tumor suppressor. Mice deficient in ZIMP10 result in embryonic lethality by E10.5; these embryos reveal severe defects in the reorganization of the yolk sac vascular plexus, indicating that ZIMP10 plays an important role in proper vascular development.