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Product Description
POFUT1 Antibody | 4765 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: Predicted species reactivity based on immunogen sequence: Rat: (94%)
Immunogen: POFUT1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human POFUT1.
The immunogen is located within amino acids 90 - 140 of POFUT1.
Research Area: Signal Transduction
Tested Application: E, WB, IHC-P
Application: POFUT1 antibody can be used for the detection of POFUT1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificiy: At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.
Positive Control 1: Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2: Cat. No. 10-501 - Human Heart Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: POFUT1 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: POFUT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: POFUT1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: POFUT1 Antibody: DDD2, FUT12, O-FUT, OFUCT1, O-Fuc-T, O-FucT-1, KIAA0180, GDP-fucose protein O-fucosyltransferase 1, Peptide-O-fucosyltransferase 1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: POFUT1 Antibody: POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling.
