Product Description
SCUBE3 Antibody | 5847 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: SCUBE3 antibody was raised against a 15 amino acid peptide near the center of human SCUBE3.
The immunogen is located within amino acids 440 - 490 of SCUBE3.
Research Area: Signal Transduction
Tested Application: E, IHC-P
Application: SCUBE3 antibody can be used for detection of SCUBE3 by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 109 kDa
Validation: N/A
Isoform: N/A
Purification: SCUBE3 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: SCUBE3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: SCUBE3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: SCUBE3 Antibody: CEGF3, CEGF3, Signal peptide, CUB and EGF-like domain-containing protein 3
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: SCUBE3 Antibody: SCUBE3 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and is highly expressed in primary osteoblasts and bones, and to a lesser extent in heart (1, 2) . Other studies have shown that overexpression of SCUBE3 in mice induced cardiac hypertrophy, suggesting that it may also play a role in the regulation of cardiac growth. SCUBE3 has been shown to be an endogenous TGF-beta receptor ligand (3, 4) and is thought to promote lung cancer cell mobility and invasiveness. In lung cancer cells, the secreted SCUBE3 protein was cleaved by MMP2 and MMP9, allowing the activation of the TGF-beta receptor, the increase of Smad2/3 transcriptional activity and the upregulation of expression of proteins such as TGF-beta1, VEGF, Snail, and Slug.