Product Description
CD10 Antibody [2A1H5E1] | 32-127 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Ni-NTA purified recombinant human CD-10 expressed in E. Coli strain BL21 (DE3) .
Research Area: Signal Transduction
Tested Application: E, IHC
Application: IHC (P) :Dilution 1:100 - 1:200
IHC (F) :Dilution 1:200 - 1:400
ELISA:Propose dilution 1:10, 000.
Determining optimal working dilutions by titration test.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by protein G affinity chromatography.
Clonality: Monoclonal
Clone: 2A1H5E1
Isotype: IgG2b
Conjugate: Unconjugated
Physical State: N/A
Buffer: Purified antibody in PBS containing 0.03% sodium azide.
Concentration: N/A
Storage Condition: CD10 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: EPN, Atriopeptidase, CALLA, NEP, SFE, CD10
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: CD10 is a 100 kDa glycoprotein, also designated Common Acute Lymphocytic Leukemia Antigen (CALLA) . It is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML) . It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.