Product Description
CD14 Antibody [5A3H5D5 (FC) / 5A3B11B5] | 32-128 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Ni-NTA purified recombinant human CD14 expressed in E. Coli strain BL21 (DE3) .
Research Area: Innate Immunity
Tested Application: E, IHC
Application: IHC:Dilution 1:200 - 1:400
ELISA:Determining optimal working dilutions by titration test.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by protein G affinity chromatography and resuspended in PBS with 0.2%BSA,
0.09% sodium azide. No reconstitution is necessar Concentration is 0.4mg/ml.
Clonality: Monoclonal
Clone: 5A3H5D5 (FC) / 5A3B11B5
Isotype: IgG1
Conjugate: Unconjugated
Physical State: N/A
Buffer: Purified antibody in PBS containing 0.03% sodium azide.
Concentration: N/A
Storage Condition: CD14 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: Myeloid cell-specific leucine-rich glycoprotein
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: CD14 antigen is a GPI-linked glycoprotein with a molecular weight of 55kD. The CD14 antigen is expressed on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. Low expression is observed on neutrophils and on human B cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding protein (LBP) . LBP and CD14 antigen serves two physiological roles. These proteins act as opsonin and opsonic receptor, respectively, to promote the phagocytic uptake of bacteria or LPScoated particles by macrophages.