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![CD32 Antibody [7.3]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/575207/811627/porsci_lo__79508.1648973713__50896.1649091856.png?c=2 "CD32 Antibody [7.3]")
![CD32 Antibody [7.3]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/575207/811627/porsci_lo__79508.1648973713__50896.1649091856.png?c=2 "CD32 Antibody [7.3]")
Product Description
CD32 Antibody [7.3] | 33-354 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: K562 and FcgRII+L cells were used as the immunogen for the CD32 antibody.
Research Area: Immunology, Signal Transduction
Tested Application: WB, Func, Flow, IF
Application: Functional Activity (Order SAF formulation)
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Optimal dilution of the CD32 antibody should be determined by the researcher.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: 7.3
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, CDw32, Fc-gamma RII-a, Fc-gamma-RIIa, FcRII-a, CD32, FCGR2A, CD32, FCG2, FCGR2A1, IGFR2
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This mAb reacts with a CD32 (FcgRII) epitope (cluster-4) . It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.
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![CD32 Antibody [8.7]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575208/811629/porsci_lo__79508.1648973713__44094.1649091857.png?c=2 "CD32 Antibody [8.7]")
