Product Description
CD34 Antibody [4H5E7 / 9B10D4] | 32-132 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Ni-NTA purified truncated recombinant CD34 expressed in E. Coli strain BL21 (DE3) .
Research Area: Immunology, Neuroscience
Tested Application: E, WB, IF
Application: Dilution
Western Blot:1:200 - 1:1, 000
IF:1:25 - 1:100
ELISA:Propose dilution 1:10, 000.
Determining optimal working dilutions by titration test.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: 4H5E7 / 9B10D4
Isotype: IgG
Conjugate: Unconjugated
Physical State: N/A
Buffer: Ascitic fluid containing 0.03% sodium azide.
Concentration: N/A
Storage Condition: CD34 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: Hematopoietic progenitor cell antigen CD34, CD_antigen=CD34
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: CD34 is a transmembrane glycoprotein with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells, endothelial cells and some fibroblasts. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells. CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.