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![CD34 Antibody [HPCA1/1171]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/575337/811915/porsci_lo__79508.1648973713__28325.1649091896.png?c=2 "CD34 Antibody [HPCA1/1171]")
![CD34 Antibody [HPCA1/1171]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/575337/811915/porsci_lo__79508.1648973713__28325.1649091896.png?c=2 "CD34 Antibody [HPCA1/1171]")
Product Description
CD34 Antibody [HPCA1/1171] | 33-500 | ProSci
Host: Mouse
Reactivity: Human, Rat
Homology: N/A
Immunogen: Recombinant full-length human protein was used as the immunogen for the anti-CD34 antibody.
Research Area: Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction
Tested Application: Flow, IF, WB, IHC-P
Application: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Western blot: 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-4 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the anti-CD34 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: HPCA1/1171
Isotype: IgG1, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Hematopoietic progenitor cell antigen CD34, CD34, CD34
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens) . Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.
![CD34 Antibody [HPCA1/1171]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/575337/811915/porsci_lo__79508.1648973713__28325.1649091896.png?c=2 "CD34 Antibody [HPCA1/1171]")
