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![CD45RA Antibody [SPM568]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/575155/811508/porsci_lo__79508.1648973713__38577.1649091842.png?c=2 "CD45RA Antibody [SPM568]")
![CD45RA Antibody [SPM568]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/575155/811508/porsci_lo__79508.1648973713__38577.1649091842.png?c=2 "CD45RA Antibody [SPM568]")
Product Description
CD45RA Antibody [SPM568] | 33-298 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Stimulated human leukocytes were used as immunogen for this anti-CD45RA antibody.
Research Area: Cancer, Immunology, Neuroscience, Signal Transduction
Tested Application: Flow, IHC, IF
Application: Flow Cytometry: 0.5-1 ug/million cells
Immunofluorescence: 1-2 ug/ml
Immunohistology (FFPE) : 0.5-1.0 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Due to variation in protocol and secondary antibody used, the anti-CD45RA antibody may need to be titered for optimal performance.
1. FFPE staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Protein G affinity chromatography
Clonality: Monoclonal
Clone: SPM568
Isotype: IgG2a, kappa
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.
Alternate Name: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
User Note: Optimal dilutions for each application to be determined by the researcher
BACKGROUND: CD45RA is an isoform of the human leukocyte common antigen (CD45) . Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve) .
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