Product Description
COX IV Antibody [1G1E1] | 13-427 | ProSci
Host: Mouse
Reactivity: Human, Mouse, Rat, Hamster, Monkey, Goat
Homology: N/A
Immunogen: A synthetic peptide of human COX IV
Research Area: Cancer, Neuroscience, Signal Transduction
Tested Application: WB, IHC, ICC, IP, Flow
Application: WB: 1:1000 - 1:4000
IHC: 1:50 - 1:200
ICC: 1:50 - 1:200
IP: 1:20 - 1:50
Flow: 1:20 - 1:50
Specificiy: N/A
Positive Control 1: LO2
Positive Control 2: U-87MG
Positive Control 3: MCF7
Positive Control 4: K-562
Positive Control 5: Jurkat
Positive Control 6: Mouse skeletal muscle
Molecular Weight: Observed: 17kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Monoclonal
Clone: [1G1E1]
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: COX4I1, COX4, COXIV, MGC72016, COX4-1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms.