Product Description
cTnI Antibody [3A10A12] | 32-141 | ProSci
Host: Mouse
Reactivity: Human
Homology: N/A
Immunogen: Ni-NTA purified truncated recombinant cTnI expressed in E. Coli strain BL21 (DE3) .
Research Area: Other
Tested Application: E, WB, IHC
Application: Western Blot:Dilution 1:500 - 1:2, 000
IHC (P) :Dilution 1:500 - 2, 000
IHC (F) :Dilution 1:500 - 2, 000
ELISA:Propose dilution 1:10, 000.
Determining optimal working dilutions by titration test.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Monoclonal
Clone: 3A10A12
Isotype: IgG1
Conjugate: Unconjugated
Physical State: N/A
Buffer: Ascitic fluid containing 0.03% sodium azide.
Concentration: N/A
Storage Condition: cTnI monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: TNNC1, Cardiac troponin ICMH7, RCM1, cTnI, CMD2A, TNNC1, CMD1FF
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: cTnI has an apparent molecular weight of 22.5 kDa. cTnI is a candidate marker with acceptable sensitivity and specificity for AMI and other cardiac diseases. Troponin, a molecule that binds to the thin filament (actin) of striated muscle fibers, acts with intracellular calcium to control the interaction of the thin filament with the thick filament (myosin) , thus regulating muscle contraction. Troponin I prevents muscle contraction in the absence of calcium, which has two skeletal muscle isoforms with considerable amino acid sequence homology. cTnI contains an additional N-terminal sequence and is highly specific for myocardium.